Life Science PhD Meeting
Summary of the 2024 Life Science PhD meeting.
Please write to phd@i-med.ac.at if you have any questions. The abstract submission Deadline is 25.01.24. Please beware that we have limited space for talks, so only the best abstracts will be granted a talk. If you are not selected for a talk, you will automatically get a poster.
Poster format is A0 (or smaller) in portrait format.
Short talk duration is 12 min + 3 min discussion.
If you are interested in the “From sample preparation to flow cytometry ” workshop organized by the Miltenyi Biotec, you can register with the same link.
We like to thank our sponsors that enabled us to make this meeting free of charge and are looking forward to see many of you at the Life Science PhD Meeting Innsbruck 2024!
Frances Ashcroft
University of Oxford
“tba“
Nageswara Rao Tata
Kantonsspital St. Gallen
“tba“
Conibear Anne Claire
TU Wien
“tba”
Marlene Bartos
University of Freiburg
“tba“
Rafael T. M. de Rosales
King’s College London
From Sample Preparation to Flow Cytometry
Flow Cytometry is a widely used technique to analyze cell populations for the expression of specific markers. A broad variety of instruments is available for almost every need.
But preparing a sample for an efficient and meaningful flow analysis can be challenging sometimes. Especially complex samples like tissues or blood products need attention as they potentially contain debris, dead cells or other factors that can lead to non-evaluable results.
Another challenge for the analysis are target cell populations with a very low frequency.
The flow analysis itself starts with the design of appropriate panels of antibodies. Choosing the best suitable antibodies and fluorochromes is a very crucial step. Compensation of the fluorescence and handling the evaluation of multi-color-panels is getting more and more important as the number of potential parameters is constantly growing.
In this workshop, we will focus on the following topics:
-Correct preparation of tissues to obtain the best viable single cell suspensions for flow cytometry applications.
-MACS separation of specific target cells as an enrichment method for rare cell populations.
-Basics of flow cytometry: Design of multicolor flow panels, setting up the compensation, antibody titrations, suitable controls, like isotypes or FMOs
Presenters: Dr. Anke Hornbach-Schäfer, Miltenyi Biotec
TBD, Miltenyi Biotec
