Life Science PhD Meeting

Life Science PhD Meeting

Summary of the 2024 Life Science PhD meeting.

Frances Ashcroft
University of Oxford

tba



Nageswara Rao Tata
Kantonsspital St. Gallen

tba




Conibear Anne Claire
TU Wien

tba



Marlene Bartos
University of Freiburg



tba




Rafael T. M. de Rosales
King’s College London




From Sample Preparation to Flow Cytometry

Flow Cytometry is a widely used technique to analyze cell populations for the expression of specific markers. A broad variety of instruments is available for almost every need.
But preparing a sample for an efficient and meaningful flow analysis can be challenging sometimes. Especially complex samples like tissues or blood products need attention as they potentially contain debris, dead cells or other factors that can lead to non-evaluable results.
Another challenge for the analysis are target cell populations with a very low frequency.
The flow analysis itself starts with the design of appropriate panels of antibodies. Choosing the best suitable antibodies and fluorochromes is a very crucial step. Compensation of the fluorescence and handling the evaluation of multi-color-panels is getting more and more important as the number of potential parameters is constantly growing.
In this workshop, we will focus on the following topics:
-Correct preparation of tissues to obtain the best viable single cell suspensions for flow cytometry applications.
-MACS separation of specific target cells as an enrichment method for rare cell populations.
-Basics of flow cytometry: Design of multicolor flow panels, setting up the compensation, antibody titrations, suitable controls, like isotypes or FMOs

Presenters: Dr. Anke Hornbach-Schäfer, Miltenyi Biotec
TBD, Miltenyi Biotec